Sprzęganie przeciwciała z polimerem wrażliwym
Adam
- 0
Sprzęganie przeciwciała z polimerem wrażliwym na temperaturę poprzez reakcję typu click on in situ w celu umożliwienia wzbogacenia biomarkerem w celu zwiększenia czułości diagnostycznej
Early analysis of infectious illnesses is likely one of the present prevalent challenges, particularly in low and restricted useful resource settings the place easy, quick, transportable, low cost, and delicate diagnostic approaches are wanted. Lateral stream immunoassay (LFIA) is a typical, speedy screening assay. Nevertheless, the low assay sensitivity limits the utility of LFIA for specimens with low pathogenic masses (early an infection levels).
Antibodies conjugated with stimulus-responsive polymers have been beforehand utilized to enhance assay sensitivity for detection of biomarkers at low concentrations. Nevertheless, the lack of antibody affinity after polymer conjugation stays a major problem. On this research, we developed poly(N-isopropylacrylamide-co-N-(2-hydroxyisopropyl)acrylamide-co-strained alkyne-isopropylacrylamide), a novel polymer for biomarker enrichment, by polymer conjugation after antibody-antigen recognition.
We employed and promoted the clicking chemistry in situ, to facilitate extremely particular conjugation between novel temperature-responsive polymers and antibody-antigen complexes. This technique might suppress the lower within the binding fixed related to polymer conjugation (>20-fold).
The conjugation was efficiently demonstrated in physique fluids comparable to urine and saliva. We achieved >5-fold antigen enrichment by way of thermal precipitation by conjugating polymers to the antibodies after antigen recognition. Concentrated biomarkers resulted in improved LFIA detection. This method can doubtlessly be utilized to enhance diagnostic exams for infectious illnesses in low and restricted useful resource settings.

PDCD6 Conjugated Antibody |
|||
C39099 | SAB | 100ul | EUR 476.4 |
PDCD2 Conjugated Antibody |
|||
C37210 | SAB | 100ul | EUR 476.4 |
PDCD1 Conjugated Antibody |
|||
C37212 | SAB | 100ul | EUR 476.4 |
PDCD6 Conjugated Antibody |
|||
C35872 | SAB | 100ul | EUR 476.4 |
PDCD4 Conjugated Antibody |
|||
C35873 | SAB | 100ul | EUR 476.4 |
PDCD6 Antibody, FITC conjugated |
|||
1-CSB-PA04874C0Rb | Cusabio |
|
|
Description: A polyclonal antibody against PDCD6. Recognizes PDCD6 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
PDCD1 Antibody, FITC conjugated |
|||
1-CSB-PA619964LC01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD1. Recognizes PDCD1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
PDCD2L Conjugated Antibody |
|||
C45665 | SAB | 100ul | EUR 476.4 |
PDCD10 Antibody, Biotin conjugated |
|||
1-CSB-PA861141HD01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD10. Recognizes PDCD10 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
PDCD6IP Conjugated Antibody |
|||
C43629 | SAB | 100ul | EUR 476.4 |
PDCD6IP Conjugated Antibody |
|||
C43906 | SAB | 100ul | EUR 476.4 |
PDCD6 Antibody, Biotin conjugated |
|||
1-CSB-PA04874D0Rb | Cusabio |
|
|
Description: A polyclonal antibody against PDCD6. Recognizes PDCD6 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
PDCD1 Antibody, Biotin conjugated |
|||
1-CSB-PA619964LD01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD1. Recognizes PDCD1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
PDCD1LG2 Conjugated Antibody |
|||
C36272 | SAB | 100ul | EUR 476.4 |
PDCD1LG2 Antibody, HRP conjugated |
|||
1-CSB-PA017667EB01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD1LG2. Recognizes PDCD1LG2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
PDCD10 Polyclonal Conjugated Antibody |
|||
C28949 | SAB | 100ul | EUR 476.4 |
PDCD1LG2 Antibody, FITC conjugated |
|||
1-CSB-PA017667EC01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD1LG2. Recognizes PDCD1LG2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
PDCD1LG2 Antibody, Biotin conjugated |
|||
1-CSB-PA017667ED01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD1LG2. Recognizes PDCD1LG2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
PDCD5 Polyclonal Conjugated Antibody |
|||
C30883 | SAB | 100ul | EUR 476.4 |
PDCD10 Polyclonal Antibody, HRP Conjugated |
|||
A57154 | EpiGentek |
|
|
PDCD10 Polyclonal Antibody, FITC Conjugated |
|||
A57155 | EpiGentek |
|
|
PDCD10 Polyclonal Antibody, Biotin Conjugated |
|||
A57156 | EpiGentek |
|
|
PDCD6 Polyclonal Antibody, HRP Conjugated |
|||
A51814 | EpiGentek |
|
|
PDCD1 Polyclonal Antibody, HRP Conjugated |
|||
A60201 | EpiGentek |
|
|
PDCD6 Polyclonal Antibody, FITC Conjugated |
|||
A51815 | EpiGentek |
|
|
PDCD1 Polyclonal Antibody, FITC Conjugated |
|||
A60200 | EpiGentek |
|
|
ALIX / PDCD6IP Polyclonal Conjugated Antibody |
|||
C30409 | SAB | 100ul | EUR 476.4 |
PDCD6 Polyclonal Antibody, Biotin Conjugated |
|||
A51816 | EpiGentek |
|
|
PDCD1 Polyclonal Antibody, Biotin Conjugated |
|||
A60199 | EpiGentek |
|
|
PDCD1LG2 Polyclonal Antibody, HRP Conjugated |
|||
A63123 | EpiGentek |
|
|
Anti-Human PDCD10 DyLight® 488 conjugated Antibody |
|||
A01879-Dyl488 | BosterBio | 100ug/vial | EUR 412.8 |
Anti-Human PDCD10 DyLight® 550 conjugated Antibody |
|||
A01879-Dyl550 | BosterBio | 100ug/vial | EUR 412.8 |
PDCD1LG2 Polyclonal Antibody, FITC Conjugated |
|||
A63124 | EpiGentek |
|
|
PDCD1LG2 Polyclonal Antibody, Biotin Conjugated |
|||
A63125 | EpiGentek |
|
|
PDCD5 (Phospho-S119) Polyclonal Conjugated Antibody |
|||
C12967 | SAB | 100ul | EUR 476.4 |
PDCD4 (Phospho-Ser67) Polyclonal Conjugated Antibody |
|||
C11925 | SAB | 100ul | EUR 476.4 |
PDCD5 (Phospho-Ser119) Polyclonal Conjugated Antibody |
|||
C12865 | SAB | 100ul | EUR 476.4 |
PDCD4 (Phospho-Ser457) Polyclonal Conjugated Antibody |
|||
C11962 | SAB | 100ul | EUR 476.4 |
PDCD7 Antibody |
|||
37209-100ul | SAB | 100ul | EUR 302.4 |
PDCD7 antibody |
|||
70R-6011 | Fitzgerald | 50 ug | EUR 560.4 |
Description: Rabbit polyclonal PDCD7 antibody raised against the N terminal of PDCD7 |
PDCD7 antibody |
|||
70R-6012 | Fitzgerald | 50 ug | EUR 560.4 |
Description: Rabbit polyclonal PDCD7 antibody raised against the middle region of PDCD7 |
PDCD7 antibody |
|||
70R-19176 | Fitzgerald | 50 ul | EUR 522 |
Description: Rabbit polyclonal PDCD7 antibody |
PDCD7 Antibody |
|||
ABD9029 | Lifescience Market | 100 ug | EUR 525.6 |
PDCD7 Antibody |
|||
1-CSB-PA990183 | Cusabio |
|
|
Description: A polyclonal antibody against PDCD7. Recognizes PDCD7 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200 |
PDCD7 Antibody |
|||
DF9029 | Affbiotech | 200ul | EUR 420 |
PDCD7 Antibody |
|||
1-CSB-PA017674GA01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCD7. Recognizes PDCD7 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
PDCD7 Antibody |
|||
1-CSB-PA695661 | Cusabio |
|
|
Description: A polyclonal antibody against PDCD7. Recognizes PDCD7 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200 |
Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated |
|||
L30211 | SAB | 1.0 ml | EUR 134.4 |
Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated |
|||
L30311 | SAB | 1.0 ml | EUR 134.4 |
Human PDCD5 Antibody (Biotin Conjugate) |
|||
32175-05121 | AssayPro | 150 ug | EUR 442.8 |
Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated |
|||
L30111 | SAB | 1.0 ml | EUR 134.4 |
anti- PDCD7 antibody |
|||
FNab06244 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against PDCD7 |
Cy3 Conjugated Avidin |
|||
BA1037 | BosterBio | 0.5ml | EUR 194.4 |
Cy3 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody |
|||
BA1031 | BosterBio | 0.5ml | EUR 218.4 |
Human PDCD5 AssayLite Antibody (RPE Conjugate) |
|||
32175-05151 | AssayPro | 150 ug | EUR 513.6 |
Human PDCD5 AssayLite Antibody (APC Conjugate) |
|||
32175-05161 | AssayPro | 150 ug | EUR 513.6 |
Cy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody |
|||
BA1032 | BosterBio | 0.5ml | EUR 218.4 |
Cy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody |
|||
BA1034 | BosterBio | 0.5ml | EUR 218.4 |
Cy3 Conjugated Rabbit Anti-Human IgG (H+L) secondary antibody |
|||
BA1035 | BosterBio | 0.5ml | EUR 218.4 |
Human PDCD5 AssayLite Antibody (FITC Conjugate) |
|||
32175-05141 | AssayPro | 150 ug | EUR 513.6 |
Human PDCD5 AssayLite Antibody (PerCP Conjugate) |
|||
32175-05171 | AssayPro | 150 ug | EUR 565.2 |
PDCD7 Polyclonal Antibody |
|||
ABP59857-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of PDCD7 from Human, Mouse. This PDCD7 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PDCD7 protein at amino acid sequence of 300-380 |
PDCD7 Polyclonal Antibody |
|||
ABP59857-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of PDCD7 from Human, Mouse. This PDCD7 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PDCD7 protein at amino acid sequence of 300-380 |
PDCD7 Polyclonal Antibody |
|||
ABP59857-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of PDCD7 from Human, Mouse. This PDCD7 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PDCD7 protein at amino acid sequence of 300-380 |
PDCD7 Polyclonal Antibody |
|||
ES9220-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against PDCD7 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
PDCD7 Polyclonal Antibody |
|||
ES9220-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against PDCD7 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
PDCL2 Antibody, HRP conjugated |
|||
1-CSB-PA836663LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCL2. Recognizes PDCL2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
PDCL2 Antibody, FITC conjugated |
|||
1-CSB-PA836663LC01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCL2. Recognizes PDCL2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
PDCL2 Antibody, Biotin conjugated |
|||
1-CSB-PA836663LD01HU | Cusabio |
|
|
Description: A polyclonal antibody against PDCL2. Recognizes PDCL2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Cy3 Conjugated anti-Rat IgG SABC Kit |
|||
SA1079 | BosterBio | 1 kit | EUR 426 |
Cy3 Conjugated anti-Goat IgG SABC Kit |
|||
SA1076 | BosterBio | 1 kit | EUR 426 |
Cy3 Conjugated anti-Mouse IgG SABC Kit |
|||
SA1072 | BosterBio | 1 kit | EUR 426 |
Cy3 Conjugated anti-Mouse IgM SABC Kit |
|||
SA1073 | BosterBio | 1 kit | EUR 450 |
Cy3 Conjugated anti-Human IgG SABC Kit |
|||
SA1078 | BosterBio | 1 kit | EUR 426 |
PDC Polyclonal Conjugated Antibody |
|||
C31552 | SAB | 100ul | EUR 476.4 |
PDC Polyclonal Conjugated Antibody |
|||
C28666 | SAB | 100ul | EUR 476.4 |
Cy3 Conjugated anti-Rabbit IgG SABC Kit |
|||
SA1074 | BosterBio | 1 kit | EUR 426 |
PDCL Polyclonal Conjugated Antibody |
|||
C28324 | SAB | 100ul | EUR 476.4 |
PDCL2 Polyclonal Conjugated Antibody |
|||
C31987 | SAB | 100ul | EUR 476.4 |
PDCL2 Polyclonal Antibody, HRP Conjugated |
|||
A60205 | EpiGentek |
|
|
PDCL2 Polyclonal Antibody, FITC Conjugated |
|||
A60204 | EpiGentek |
|
|
Programmed Cell Death 7 (PDCD7) Antibody |
|||
abx236244-100ug | Abbexa | 100 ug | EUR 577.2 |
Programmed Cell Death 7 (PDCD7) Antibody |
|||
20-abx212468 | Abbexa |
|
|
Programmed Cell Death 7 (PDCD7) Antibody |
|||
20-abx212469 | Abbexa |
|
|
Programmed Cell Death 7 (PDCD7) Antibody |
|||
20-abx114701 | Abbexa |
|
|
Programmed Cell Death 7 (PDCD7) Antibody |
|||
abx027477-400ul | Abbexa | 400 ul | EUR 627.6 |
Programmed Cell Death 7 (PDCD7) Antibody |
|||
abx027477-80l | Abbexa | 80 µl | EUR 343.2 |
Programmed Cell Death 7 (PDCD7) Antibody |
|||
20-abx126336 | Abbexa |
|
|
PDCL2 Polyclonal Antibody, Biotin Conjugated |
|||
A60203 | EpiGentek |
|
|
CS Conjugated Antibody |
|||
C43035 | SAB | 100ul | EUR 476.4 |
KL Conjugated Antibody |
|||
C43404 | SAB | 100ul | EUR 476.4 |
XK Conjugated Antibody |
|||
C43438 | SAB | 100ul | EUR 476.4 |
Rb Conjugated Antibody |
|||
C48804 | SAB | 100ul | EUR 476.4 |
C3 Conjugated Antibody |
|||
C49413 | SAB | 100ul | EUR 476.4 |
C4 Conjugated Antibody |
|||
C49604 | SAB | 100ul | EUR 476.4 |
F8 Conjugated Antibody |
|||
C38232 | SAB | 100ul | EUR 476.4 |
F3 Conjugated Antibody |
|||
C38235 | SAB | 100ul | EUR 476.4 |
GC Conjugated Antibody |
|||
C38683 | SAB | 100ul | EUR 476.4 |
F2 Conjugated Antibody |
|||
C40244 | SAB | 100ul | EUR 476.4 |
TG Conjugated Antibody |
|||
C40380 | SAB | 100ul | EUR 476.4 |
F5 Conjugated Antibody |
|||
C45256 | SAB | 100ul | EUR 476.4 |
Ob Conjugated Antibody |
|||
C45370 | SAB | 100ul | EUR 476.4 |
ST Conjugated Antibody |
|||
C45395 | SAB | 100ul | EUR 476.4 |
CP Conjugated Antibody |
|||
C45875 | SAB | 100ul | EUR 476.4 |
C7 Conjugated Antibody |
|||
C46376 | SAB | 100ul | EUR 476.4 |
GK Conjugated Antibody |
|||
C36507 | SAB | 100ul | EUR 476.4 |
TF Conjugated Antibody |
|||
C36753 | SAB | 100ul | EUR 476.4 |
TH Conjugated Antibody |
|||
C37059 | SAB | 100ul | EUR 476.4 |
FC Conjugated Antibody |
|||
C48379 | SAB | 100ul | EUR 476.4 |
T Conjugated Antibody |
|||
C48451 | SAB | 100ul | EUR 476.4 |
Metoda selektywnej koniugacji N-końca poszerza okno terapeutyczne koniugatów przeciwciało-lek poprzez poprawę tolerancji i stabilności
Antibody-drug conjugates (ADCs) are focused therapeutic brokers that deal with cancers by selective supply of extremely potent cytotoxic medicine to tumor cells by way of cancer-specific antibodies. Nevertheless, their medical profit is restricted by off-target toxicity and slim therapeutic home windows. To beat these limitations, now we have utilized reductive alkylation to develop a brand new kind of ADC that has cytotoxic medicine conjugated to the N-terminal of an antibody by way of amine bonds launched by way of reductive alkylation reactions (NTERM). To check whether or not the NTERM-conjugated ADCs can widen therapeutic home windows, we synthesized three totally different ADCs by conjugating trastuzumab and monomethyl auristatin-F utilizing three totally different strategies, and in contrast their stability, efficacy, and toxicity.
The NTERM-conjugated ADC was extra steady in vitro and in vivo than the thiol-conjugated and the lysine-conjugated ADCs. The NTERM-conjugated ADC confirmed decrease toxicity in comparison with different ADCs, whereas its efficacy was akin to that of the thiol-conjugated ADC and higher than that of the lysine-conjugated ADC. These outcomes recommend that the NTERM conjugation technique might widen the therapeutic window of ADCs by enhancing its stability and lowering toxicity.
Uniwersalny check immunologiczny ze znacznikiem SERS do wykrywania bakterii chorobotwórczych w oparciu o separację Fe 3 O 4 @ Au-aptamer i rozpoznawanie orientacji przeciwciało-białko A
Speedy, dependable and delicate detection strategies for pathogenic micro organism are strongly demanded. Herein, we proposed a magnetically assisted floor enhanced Raman scattering (SERS)-label immunoassay for the delicate detection of micro organism by utilizing a common method based mostly on free antibody labelling and staphylococcus proteins A (PA)-SERS tags orientation recognition. The SERS biosensor consists of two purposeful nanomaterials: aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) as magnetic SERS platform for pathogen enrichment and PA modified-SERS tags (Au@DTNB@PA) as a common probe for goal micro organism quantitative detection. After goal micro organism enriched, free antibody was used to particular marking goal micro organism and supplied quite a few Fc fragment, which might information the PA-SERS tags orientation-dependent binding.
With this technique, Fe3O4@Au/micro organism/SERS tags sandwich immunocomplexes for many micro organism (anticipate a number of species of Staphylococcus) had been straightforward constructed. The bounds of detection (LODs) of the proposed assay had been discovered to be 10, 10, and 25 cells/mL for 3 widespread pathogens Escherichia coli (E. coli), Listeria monocytogenes (L. mono), and Salmonella typhimurium (S. typhi), respectively, in actual meals samples. The common technique additionally displays the benefits of speedy, sturdy, and straightforward to function, suggesting its nice potential for meals security monitoring and infectious illnesses analysis.
Zwiększenie wydajności poprzez tworzenie wzoru składania gradientu cząstek immunosensora elektrochemiluminescencyjnego utworzonego za pomocą magnetolitografii w oznaczaniu albuminy surowicy ludzkiej
Gradient properties facilitate the correlation of chemical and bodily options of particles on the construction and adherent destiny. Herein, efficiency enhancement is explored by particle gradient meeting patterning (PGAP) shaped with magnetic discipline gradient (MFG) by magnetolithography (ML) within the electrochemiluminescence (ECL) measurement. Magnetic Fe3O4 nanoparticles had been chosen as nanocarriers and coated with a SiO2 layer for immobilization of main antibodies. CdTe quantum dots with protein G coatings had been chosen as sign labels and conjugated with secondary antibodies. Magnetized 500-nm pillar, 1 μm- and three μm-line arrays of nickel had been positioned behind the working electrode modifying the sandwich-structured ECL immunosensor to type numerous PGAPs.
A efficiency enhancement of ca. 2.Four instances was noticed when evaluating the PGAP-free immunosensor to the researched gradient immunosensor, shaped with a magnetized Three μm-line array of nickel. This concludes that the sensitivity of an ECL immunosensor has been enhanced as a result of PGAP properties. When the immunosensor with PGAP properties was used to quantify human serum albumin, it exhibited a large linear vary (10-480 ng/mL), and a restrict of detection of 10 ng/mL. PGAP properties, shaped with MFG by ML, supplies a easy technique to enhance the ECL efficiency.
Ocena [Cys (ATTO 488) 8] Dermorphin-NH2 jako nowego narzędzia do badania receptorów peptydowych opioidowych μ
The μ-opioid peptide (MOP) receptor is a member of the opioid receptor household and an vital medical goal for analgesia. Measuring MOP receptor location and monitoring its turnover historically used radiolabels or antibodies with attendant issues of utility of radiolabels in complete cells and poor antibody selectivity. To deal with these points now we have synthesized and characterised a novel ATTO488 based mostly fluorescent Dermorphin analogue; [Cys(ATTO 488)8]Dermorphin-NH2 (DermATTO488).
We initially assessed the binding profile of DermATTO488 in HEK cells expressing human MOP and CHO cells expressing human MOP, δ-opioid peptide (DOP), κ-opioid peptide (KOP) and Nociceptin/Orphanin FQ peptide (NOP) receptors utilizing radioligand binding. Purposeful exercise of the conjugated peptide was assessed by measuring (i) the flexibility of the ligand to have interaction G-protein by measuring the flexibility to stimulate GTPγ[35S] binding and (ii) the flexibility to stimulate phosphorylation of ERK1/2. Receptor location was visualised utilizing confocal scanning laser microscopy.
Dermorphin and DermATTO488 sure to HEKMOP (pKi: 8.29 and seven.00; p<0.05), CHOMOP (pKi: 9.26 and eight.12; p<0.05) and CHODOP (pKi: 7.03 and seven.16; p>0.05). Each ligands had been inactive at KOP and NOP. Dermorphin and DermATTO488 stimulated the binding of GTPγ[35S] with comparable pEC50 (7.84 and seven.62; p>0.05) and Emax (1.52 and 1.34fold p>0.05) values. Furthermore, Dermorphin and DermATTO488 produced a monophasic stimulation of ERK1/2 phosphorylation peaking at 5mins (6.98 and seven.64-fold; p>0.05).
Lastly, in confocal microscopy DermATTO488 sure to recombinant MOP receptors on CHO and HEK cells in a focus dependent method that might be blocked by pre-incubation with unlabelled Dermorphin or Naloxone. Collectively, addition to ATTO488 to Dermorphin produced a ligand not dissimilar to Dermorphin; with ~10fold selectivity over DOP. This new ligand DermATTO488 retained purposeful exercise and might be used to visualise MOP receptor location.
CXXC5 Antibody |
|||
6111-002mg | ProSci | 0.02 mg | EUR 206.18 |
Description: CXXC5 Antibody: CXXC5 is zinc finger protein closely related to the DVL1-binding protein CXXC4 (also known as Idax), and like CXXC4 is thought to modulate the Wnt signaling pathway during development. CXXC5 is upregulated in neural stem cells upon BMP4 stimulation, leading to decreased levels of the Wnt-signaling target AXIN2 and facilitated the response of the stem cells to Wnt3a. Recent reports suggest CXXC5 transcription is activated by the Wilms tumor 1 (WT1) transcription factor, and together are involved in the WNT/beta-catenin pathway and play a role in embryonic kidney development. |
CXXC5 Antibody |
|||
6111-01mg | ProSci | 0.1 mg | EUR 523.7 |
Description: CXXC5 Antibody: CXXC5 is zinc finger protein closely related to the DVL1-binding protein CXXC4 (also known as Idax), and like CXXC4 is thought to modulate the Wnt signaling pathway during development. CXXC5 is upregulated in neural stem cells upon BMP4 stimulation, leading to decreased levels of the Wnt-signaling target AXIN2 and facilitated the response of the stem cells to Wnt3a. Recent reports suggest CXXC5 transcription is activated by the Wilms tumor 1 (WT1) transcription factor, and together are involved in the WNT/beta-catenin pathway and play a role in embryonic kidney development. |
CXXC5 Antibody |
|||
36381-100ul | SAB | 100ul | EUR 302.4 |
CXXC5 Antibody |
|||
DF12378 | Affbiotech | 200ul | EUR 420 |
CXXC5 Antibody |
|||
1-CSB-PA806953 | Cusabio |
|
|
Description: A polyclonal antibody against CXXC5. Recognizes CXXC5 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200 |
CXXC5 Antibody |
|||
1-CSB-PA565730 | Cusabio |
|
|
Description: A polyclonal antibody against CXXC5. Recognizes CXXC5 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:200-1:1000, IHC:1:50-1:200 |
CXXC5 Antibody |
|||
1-CSB-PA741975LA01HU | Cusabio |
|
|
Description: A polyclonal antibody against CXXC5. Recognizes CXXC5 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
CS Antibody, HRP conjugated |
|||
1-CSB-PA006031LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against CS. Recognizes CS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
TG Antibody, HRP conjugated |
|||
1-CSB-PA12325B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against TG. Recognizes TG from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
KL Antibody, HRP conjugated |
|||
1-CSB-PA12729B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against KL. Recognizes KL from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
CP Antibody, HRP conjugated |
|||
1-CSB-PA14599B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against CP. Recognizes CP from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
HP Antibody, HRP conjugated |
|||
1-CSB-PA14609B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against HP. Recognizes HP from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
G Antibody, HRP conjugated |
|||
1-CSB-PA14899B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against G. Recognizes G from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
Hp Antibody, HRP conjugated |
|||
1-CSB-PA17069B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against Hp. Recognizes Hp from Rat. This antibody is HRP conjugated. Tested in the following application: ELISA |
AR Antibody, HRP conjugated |
|||
1-CSB-PA05979B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against AR. Recognizes AR from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
C5 Antibody, HRP conjugated |
|||
1-CSB-PA003995LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against C5. Recognizes C5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
C5 Antibody, HRP conjugated |
|||
1-CSB-PA003995YB01PI | Cusabio |
|
|
Description: A polyclonal antibody against C5. Recognizes C5 from Pig. This antibody is HRP conjugated. Tested in the following application: ELISA |
C6 Antibody, HRP conjugated |
|||
1-CSB-PA004036LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against C6. Recognizes C6 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
C9 Antibody, HRP conjugated |
|||
1-CSB-PA004259LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against C9. Recognizes C9 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
Tf Antibody, HRP conjugated |
|||
1-CSB-PA00470F0Rb | Cusabio |
|
|
Description: A polyclonal antibody against Tf. Recognizes Tf from Rat. This antibody is HRP conjugated. Tested in the following application: ELISA |
gD Antibody, HRP conjugated |
|||
1-CSB-PA18929B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against gD. Recognizes gD from Human herpesvirus 1. This antibody is HRP conjugated. Tested in the following application: ELISA |
HA Antibody, HRP conjugated |
|||
1-CSB-PA18939B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against HA. Recognizes HA from Influenza A virus(H7N9). This antibody is HRP conjugated. Tested in the following application: ELISA |
th Antibody, HRP conjugated |
|||
1-CSB-PA24419B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against th. Recognizes th from Zebrafish. This antibody is HRP conjugated. Tested in the following application: ELISA |
L1 Antibody, HRP conjugated |
|||
1-CSB-PA08349B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against L1. Recognizes L1 from Human papillomavirus type 16. This antibody is HRP conjugated. Tested in the following application: ELISA |
L1 Antibody, HRP conjugated |
|||
1-CSB-PA08359B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against L1. Recognizes L1 from Human papillomavirus type 18. This antibody is HRP conjugated. Tested in the following application: ELISA |
C3 Antibody, HRP conjugated |
|||
1-CSB-PA10599B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against C3. Recognizes C3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
GK Antibody, HRP conjugated |
|||
1-CSB-PA300097LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against GK. Recognizes GK from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
IX Antibody, HRP conjugated |
|||
1-CSB-PA301675LB01ECY | Cusabio |
|
|
Description: A polyclonal antibody against IX. Recognizes IX from Enterobacteria phage M13. This antibody is HRP conjugated. Tested in the following application: ELISA |
P Antibody, HRP conjugated |
|||
1-CSB-PA303154LB01RAH | Cusabio |
|
|
Description: A polyclonal antibody against P. Recognizes P from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
GP Antibody, HRP conjugated |
|||
1-CSB-PA308957LB01ZAA | Cusabio |
|
|
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
GP Antibody, HRP conjugated |
|||
1-CSB-PA310843LB01ZAA | Cusabio |
|
|
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
GP Antibody, HRP conjugated |
|||
1-CSB-PA314099LB01ZAT | Cusabio |
|
|
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
F Antibody, HRP conjugated |
|||
1-CSB-PA318261LB01CCQ | Cusabio |
|
|
Description: A polyclonal antibody against F. Recognizes F from Canine distemper virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
N Antibody, HRP conjugated |
|||
1-CSB-PA318401LB01LNP | Cusabio |
|
|
Description: A polyclonal antibody against N. Recognizes N from Lassa virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
N Antibody, HRP conjugated |
|||
1-CSB-PA318492LB01JAM | Cusabio |
|
|
Description: A polyclonal antibody against N. Recognizes N from Junin mammarenavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
gH Antibody, HRP conjugated |
|||
1-CSB-PA319015LB01HWV | Cusabio |
|
|
Description: A polyclonal antibody against gH. Recognizes gH from Human cytomegalovirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
N Antibody, HRP conjugated |
|||
1-CSB-PA321352LB01RAI | Cusabio |
|
|
Description: A polyclonal antibody against N. Recognizes N from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
N Antibody, HRP conjugated |
|||
1-CSB-PA321352YB01RAI | Cusabio |
|
|
Description: A polyclonal antibody against N. Recognizes N from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
M Antibody, HRP conjugated |
|||
1-CSB-PA322192LB01RAI | Cusabio |
|
|
Description: A polyclonal antibody against M. Recognizes M from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
S Antibody, HRP conjugated |
|||
1-CSB-PA322803LB01BJK | Cusabio |
|
|
Description: A polyclonal antibody against S. Recognizes S from Bovine coronavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
C Antibody, HRP conjugated |
|||
1-CSB-PA323345LB01RAI | Cusabio |
|
|
Description: A polyclonal antibody against C. Recognizes C from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
X Antibody, HRP conjugated |
|||
1-CSB-PA323774LB01WAW | Cusabio |
|
|
Description: A polyclonal antibody against X. Recognizes X from Woodchuck hepatitis B virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
P Antibody, HRP conjugated |
|||
1-CSB-PA324598LB01HDO | Cusabio |
|
|
Description: A polyclonal antibody against P. Recognizes P from Hepatitis B virus genotype A2 subtype adw2. This antibody is HRP conjugated. Tested in the following application: ELISA |
GC Antibody, HRP conjugated |
|||
1-CSB-PA07569B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against GC. Recognizes GC from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
MB Antibody, HRP conjugated |
|||
1-CSB-PA07917B0Rb | Cusabio |
|
|
Description: A polyclonal antibody against MB. Recognizes MB from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
X Antibody, HRP conjugated |
|||
1-CSB-PA865559LB01HEO | Cusabio |
|
|
Description: A polyclonal antibody against X. Recognizes X from Hepatitis B virus genotype D subtype ayw. This antibody is HRP conjugated. Tested in the following application: ELISA |
NP Antibody, HRP conjugated |
|||
1-CSB-PA841983HB01RCI | Cusabio |
|
|
Description: A polyclonal antibody against NP. Recognizes NP from Reston ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
B1 Antibody, HRP conjugated |
|||
1-CSB-PA882501LB01HKA | Cusabio |
|
|
Description: A polyclonal antibody against B1. Recognizes B1 from Human herpesvirus 6B. This antibody is HRP conjugated. Tested in the following application: ELISA |
L Antibody, HRP conjugated |
|||
1-CSB-PA806333LB01VBE | Cusabio |
|
|
Description: A polyclonal antibody against L. Recognizes L from Vesicular stomatitis Indiana virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
FH Antibody, HRP conjugated |
|||
1-CSB-PA008659LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against FH. Recognizes FH from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
PC Antibody, HRP conjugated |
|||
1-CSB-PA017511LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against PC. Recognizes PC from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
XG Antibody, HRP conjugated |
|||
1-CSB-PA026190LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against XG. Recognizes XG from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
F2 Antibody, HRP conjugated |
|||
1-CSB-PA007923LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against F2. Recognizes F2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
F3 Antibody, HRP conjugated |
|||
1-CSB-PA007928LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against F3. Recognizes F3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
F5 Antibody, HRP conjugated |
|||
1-CSB-PA007929HB01HU | Cusabio |
|
|
Description: A polyclonal antibody against F5. Recognizes F5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
F7 Antibody, HRP conjugated |
|||
1-CSB-PA007930HB01HU | Cusabio |
|
|
Description: A polyclonal antibody against F7. Recognizes F7 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
F8 Antibody, HRP conjugated |
|||
1-CSB-PA007932LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against F8. Recognizes F8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
F9 Antibody, HRP conjugated |
|||
1-CSB-PA007936LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against F9. Recognizes F9 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
TH Antibody, HRP conjugated |
|||
1-CSB-PA023470LB01HU | Cusabio |
|
|
Description: A polyclonal antibody against TH. Recognizes TH from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
TK Antibody, HRP conjugated |
|||
1-CSB-PA023577HB01HWY | Cusabio |
|
|
Description: A polyclonal antibody against TK. Recognizes TK from Human herpesvirus 1. This antibody is HRP conjugated. Tested in the following application: ELISA |
MB Antibody, HRP conjugated |
|||
1-CSB-PA013529YB01HU | Cusabio |
|
|
Description: A polyclonal antibody against MB. Recognizes MB from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
AK Antibody, HRP conjugated |
|||
1-CSB-PA511311HB01ETF | Cusabio |
|
|
Description: A polyclonal antibody against AK. Recognizes AK from Penaeus monodon. This antibody is HRP conjugated. Tested in the following application: ELISA |
gL Antibody, HRP conjugated |
|||
1-CSB-PA515269LB01HWW | Cusabio |
|
|
Description: A polyclonal antibody against gL. Recognizes gL from Human cytomegalovirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
X Antibody, HRP conjugated |
|||
1-CSB-PA526046LB01HVP | Cusabio |
|
|
Description: A polyclonal antibody against X. Recognizes X from Hepatitis B virus genotype D. This antibody is HRP conjugated. Tested in the following application: ELISA |
NP Antibody, HRP conjugated |
|||
1-CSB-PA529597LB01IMP | Cusabio |
|
|
Description: A polyclonal antibody against NP. Recognizes NP from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
P Antibody, HRP conjugated |
|||
1-CSB-PA768100LB01VBH | Cusabio |
|
|
Description: A polyclonal antibody against P. Recognizes P from Vesicular stomatitis Indiana virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
NS Antibody, HRP conjugated |
|||
1-CSB-PA604972LB01IER | Cusabio |
|
|
Description: A polyclonal antibody against NS. Recognizes NS from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
HA Antibody, HRP conjugated |
|||
1-CSB-PA607773LB01IER | Cusabio |
|
|
Description: A polyclonal antibody against HA. Recognizes HA from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
NP Antibody, HRP conjugated |
|||
1-CSB-PA325769LB01IIM | Cusabio |
|
|
Description: A polyclonal antibody against NP. Recognizes NP from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
S Antibody, HRP conjugated |
|||
1-CSB-PA333052LB01BJO | Cusabio |
|
|
Description: A polyclonal antibody against S. Recognizes S from Bovine coronavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
E6 Antibody, HRP conjugated |
|||
1-CSB-PA334186LB01HNY | Cusabio |
|
|
Description: A polyclonal antibody against E6. Recognizes E6 from Human papillomavirus type 52. This antibody is HRP conjugated. Tested in the following application: ELISA |
N Antibody, HRP conjugated |
|||
1-CSB-PA334828LB01PXE | Cusabio |
|
|
Description: A polyclonal antibody against N. Recognizes N from Puumala virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
S Antibody, HRP conjugated |
|||
1-CSB-PA336163EB01HIY | Cusabio |
|
|
Description: A polyclonal antibody against S. Recognizes S from Human coronavirus OC43. This antibody is HRP conjugated. Tested in the following application: ELISA |
GP Antibody, HRP conjugated |
|||
1-CSB-PA350772LB01ZAT | Cusabio |
|
|
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA |
X Antibody, HRP conjugated |
|||
1-CSB-PA355969LB01HEM | Cusabio |
|
|
Description: A polyclonal antibody against X. Recognizes X from Hepatitis B virus genotype D subtype ayw. This antibody is HRP conjugated. Tested in the following application: ELISA |
F Antibody, HRP conjugated |
|||
1-CSB-PA356041LB01HTO | Cusabio |
|
|
Description: A polyclonal antibody against F. Recognizes F from Human respiRatory syncytial virus A. This antibody is HRP conjugated. Tested in the following application: ELISA |
NP Antibody, HRP conjugated |
|||
1-CSB-PA356056LB01IFZ | Cusabio |
|
|
Description: A polyclonal antibody against NP. Recognizes NP from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
NS Antibody, HRP conjugated |
|||
1-CSB-PA356065LB01IIE | Cusabio |
|
|
Description: A polyclonal antibody against NS. Recognizes NS from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
HA Antibody, HRP conjugated |
|||
1-CSB-PA356317LB01IDG | Cusabio |
|
|
Description: A polyclonal antibody against HA. Recognizes HA from Influenza A virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
NP Antibody, HRP conjugated |
|||
1-CSB-PA356318HB01IJU | Cusabio |
|
|
Description: A polyclonal antibody against NP. Recognizes NP from Influenza B virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
L2 Antibody, HRP conjugated |
|||
1-CSB-PA356939LB01HMN | Cusabio |
|
|
Description: A polyclonal antibody against L2. Recognizes L2 from Human papillomavirus type 18. This antibody is HRP conjugated. Tested in the following application: ELISA |
C3 Antibody, HRP conjugated |
|||
1-CSB-PA360475HB01RA | Cusabio |
|
|
Description: A polyclonal antibody against C3. Recognizes C3 from Rat. This antibody is HRP conjugated. Tested in the following application: ELISA |
NP Antibody, HRP conjugated |
|||
1-CSB-PA361330LB01IJK | Cusabio |
|
|
Description: A polyclonal antibody against NP. Recognizes NP from Influenza B virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
gG Antibody, HRP conjugated |
|||
1-CSB-PA361844HB01HWY | Cusabio |
|
|
Description: A polyclonal antibody against gG. Recognizes gG from Human herpesvirus 1. This antibody is HRP conjugated. Tested in the following application: ELISA |
E7 Antibody, HRP conjugated |
|||
1-CSB-PA361948LB01HMN | Cusabio |
|
|
Description: A polyclonal antibody against E7. Recognizes E7 from Human papillomavirus type 18. This antibody is HRP conjugated. Tested in the following application: ELISA |
E2 Antibody, HRP conjugated |
|||
1-CSB-PA361949LB01HMN | Cusabio |
|
|
Description: A polyclonal antibody against E2. Recognizes E2 from Human papillomavirus type 18. This antibody is HRP conjugated. Tested in the following application: ELISA |
gB Antibody, HRP conjugated |
|||
1-CSB-PA362479LB01HJX | Cusabio |
|
|
Description: A polyclonal antibody against gB. Recognizes gB from Human herpesvirus 2. This antibody is HRP conjugated. Tested in the following application: ELISA |
C3 Antibody, HRP conjugated |
|||
1-CSB-PA365507LB01MO | Cusabio |
|
|
Description: A polyclonal antibody against C3. Recognizes C3 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA |
L2 Antibody, HRP conjugated |
|||
1-CSB-PA365848LB01HML | Cusabio |
|
|
Description: A polyclonal antibody against L2. Recognizes L2 from Human papillomavirus type 16. This antibody is HRP conjugated. Tested in the following application: ELISA |
E6 Antibody, HRP conjugated |
|||
1-CSB-PA365854LB01HML | Cusabio |
|
|
Description: A polyclonal antibody against E6. Recognizes E6 from Human papillomavirus type 16. This antibody is HRP conjugated. Tested in the following application: ELISA |