• 19 maja, 2021
bodiesrevealed

Białko mitochondrialne CMPK2 reguluje tworzenie

Białko mitochondrialne CMPK2 reguluje tworzenie komórek piankowatych wzmocnionych przez IFN alfa, potencjalnie przyczyniając się do przedwczesnej miażdżycy tętnic w SLE 

Background: Untimely atherosclerosis happens in sufferers with SLE; nonetheless, the mechanisms stay unclear. Each mitochondrial equipment and proinflammatory cytokine interferon alpha (IFN-α) probably contribute to atherogenic processes in SLE. Right here, we discover the roles of the mitochondrial protein cytidine/uridine monophosphate kinase 2 (CMPK2) in IFN-α-mediated pro-atherogenic occasions.

Strategies: Foam cell measurements have been carried out by oil purple O staining, Dil-oxLDL uptake and the BODIPY strategy. The mRNA and protein ranges have been measured by qPCR and Western blotting, respectively. Isolation of CD4+ T cells and monocytes was carried out with monoclonal antibodies conjugated with microbeads. Manipulation of protein expression was performed by both small interference RNA (siRNA) knockdown or CRISPR/Cas9 knockout. The expression of mitochondrial reactive oxygen species (mtROS) was decided by circulate cytometry and confocal microscopy.

Outcomes: IFN-α enhanced oxLDL-induced foam cell formation and Dil-oxLDL uptake by macrophages. Along with IFN-α, a number of triggers of atherosclerosis, together with thrombin and IFN-γ, can induce CMPK2 expression, which was elevated in CD4+ T cells and CD14+ monocytes remoted from SLE sufferers in comparison with these remoted from controls. The evaluation of mobile subfractions revealed that CMPK2 was current in each mitochondrial and cytosolic fractions. IFN-α-induced CMPK2 expression was inhibited by Janus kinase (JAK)half of and tyrosine kinase 2 (Tyk2) inhibitors. Each the knockdown and knockout of CMPK2 attenuated IFN-α-mediated foam cell formation, which concerned the discount of scavenger receptor class A (SR-A) expression. CMPK2 additionally regulated IFN-α-enhanced mtROS manufacturing and inflammasome activation.

Conclusions: The research means that CMPK2 performs contributing roles within the pro-atherogenic results of IFN-α.

bodiesrevealed
bodiesrevealed

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6C2 (formerly 6C2)

TA503086 100 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6C2 (formerly 6C2)

TA503086S 30 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI8E3 (formerly 8E3)

TA503110 100 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI8E3 (formerly 8E3)

TA503110S 30 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)

TA503130 100 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)

TA503130S 30 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)

TA502962 100 µl Ask for price

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)

TA502962S 30 µl Ask for price

TUBB4A (untagged)-Human tubulin, beta 4 (TUBB4)

SC116314 10 µg Ask for price

TUBB4A (untagged)-Human tubulin, beta 4 (TUBB4)

SC323934 10 µg Ask for price

Human Tubulin Beta 4(TUBb4)ELISA Kit

QY-E01098 96T
EUR 433.2

Tubb4a (GFP-tagged) - Mouse tubulin, beta 4 (Tubb4)

MG207086 10 µg Ask for price

TUBB4A (GFP-tagged) - Human tubulin, beta 4 (TUBB4)

RG203945 10 µg Ask for price

Tubb4a (untagged) - Mouse tubulin, beta 4 (Tubb4), (10ug)

MC202698 10 µg Ask for price

Human Tubulin beta-4 chain (TUBB4) ELISA Kit

AE12787HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human Tubulin beta-4 chain (TUBB4) ELISA Kit

AE12787HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Human Tubulin beta-4 chain (TUBB4) ELISA Kit

KTE60116-96T5 96 T×5
EUR 2629
Description: This Human Tubulin beta-4 chain (TUBB4) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBB4.

Human Tubulin beta-4 chain (TUBB4) ELISA Kit

KTE60116-96T50 96 T×50
EUR 24569
Description: This Human Tubulin beta-4 chain (TUBB4) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBB4.

Mouse Tubulin beta- 4 chain, Tubb4 ELISA KIT

ELI-53137m 96 Tests
EUR 1038

Human Tubulin beta- 4 chain, TUBB4 ELISA KIT

ELI-13731h 96 Tests
EUR 988.8

Tubb4a (Myc-DDK-tagged) - Mouse tubulin, beta 4 (Tubb4)

MR207086 10 µg Ask for price

TUBB4A (Myc-DDK-tagged)-Human tubulin, beta 4 (TUBB4)

RC203945 10 µg Ask for price

Bovine Tubulin beta- 4 chain, TUBB4 ELISA KIT

ELI-29137b 96 Tests
EUR 1113.6

Tubb4a (untagged ORF) - Rat tubulin, beta 4 (Tubb4), (10 ug)

RN203612 10 µg Ask for price

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Tubb4a (Myc-DDK-tagged ORF) - Rat tubulin, beta 4 (Tubb4), (10 ug)

RR203612 10 µg Ask for price

Lenti ORF clone of Human tubulin, beta 4 (TUBB4), mGFP tagged

RC203945L2 10 µg Ask for price

Lenti ORF clone of Human tubulin, beta 4 (TUBB4), mGFP tagged

RC203945L4 10 µg Ask for price

Lenti ORF clone of Tubb4a (mGFP-tagged) - Mouse tubulin, beta 4 (Tubb4)

MR207086L4 10 µg Ask for price

Lenti ORF clone of Tubb4a (Myc-DDK-tagged) - Mouse tubulin, beta 4 (Tubb4)

MR207086L3 10 µg Ask for price

Beta Tubulin Antibody / TUBB3

V7658-100UG 100 ug
EUR 349.3
Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Beta Tubulin Antibody / TUBB3

V7658-20UG 20 ug
EUR 153.3
Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Beta Tubulin Antibody / TUBB3

V7658SAF-100UG 100 ug
EUR 349.3
Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Beta Tubulin Antibody / TUBB3

V7659-100UG 100 ug
EUR 349.3
Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Beta Tubulin Antibody / TUBB3

V7659-20UG 20 ug
EUR 153.3
Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Beta Tubulin Antibody / TUBB3

V7659SAF-100UG 100 ug
EUR 349.3
Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Beta Tubulin Antibody / TUBB3

V8309-100UG 100 ug
EUR 349.3
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. [UniProt]

Beta Tubulin Antibody / TUBB3

V8309-20UG 20 ug
EUR 153.3
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. [UniProt]

Beta Tubulin Antibody / TUBB3

V8309SAF-100UG 100 ug
EUR 349.3
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. [UniProt]

Beta Tubulin Antibody / TUBB1

RQ5777 100 ug
EUR 356.15
Description: TUBB1 is a gene that codes for the protein Tubulin beta-1 chain in humans. This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.

Lenti ORF clone of Human tubulin, beta 4 (TUBB4), Myc-DDK-tagged

RC203945L1 10 µg Ask for price

Lenti ORF clone of Human tubulin, beta 4 (TUBB4), Myc-DDK-tagged

RC203945L3 10 µg Ask for price

3`UTR clone of tubulin beta 4 (TUBB4) for miRNA target validation

SC210709 10 µg Ask for price

Lenti ORF clone of Tubb4a (mGFP-tagged ORF) - Rat tubulin, beta 4 (Tubb4), (10 ug)

RR203612L4 10 µg Ask for price

beta I Tubulin (TUBB1) Antibody

20-abx219168
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody

20-abx318661
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

20-abx134678
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta 3 (TUBB3) Antibody

20-abx134679
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta 3 (TUBB3) Antibody

20-abx134680
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta 3 (TUBB3) Antibody

20-abx134681
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta 3 (TUBB3) Antibody

20-abx134683
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta 3 (TUBB3) Antibody

20-abx242446
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Tubulin Beta 1 (TUBB1) Antibody

abx224141-100ug 100 ug
EUR 493.2

Tubulin Beta 3 (TUBB3) Antibody

20-abx110304
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

20-abx110305
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody

20-abx110582
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBb3) Antibody

20-abx174960
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Tubulin Beta 1 (TUBb1) Antibody

20-abx178748
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Tubulin Beta 3 (TUBB3) Antibody

20-abx121803
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin, Beta 3 (TUBB3) Antibody

20-abx116351
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Tubulin Beta 3 (TUBB3) Antibody

abx332407-100ul 100 ul
EUR 510

Tubulin Beta 3 (TUBB3) Antibody

20-abx330237
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

abx331438-100ul 100 ul
EUR 510

Tubulin Beta 3 (TUBB3) Antibody

20-abx326995
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

20-abx326998
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Tubulin Beta 6 (TUBB6) Antibody

abx026508-400ul 400 ul
EUR 627.6

Tubulin Beta 6 (TUBB6) Antibody

abx026508-80l 80 µl
EUR 343.2

Tubulin Beta 3 (TUBB3) Antibody

abx032605-400ul 400 ul
EUR 627.6

Tubulin Beta 3 (TUBB3) Antibody

abx032605-80l 80 µl
EUR 343.2

Tubulin Beta 3 (TUBB3) Antibody

abx036052-100ug 100 ug
EUR 469.2

Tubulin Beta 3 (TUBB3) Antibody

abx019140-100ug 100 ug
EUR 410.4

Tubulin Beta 3 (TUBB3) Antibody

abx019141-100ug 100 ug
EUR 410.4

Tubulin Beta 1 (TUBB1) Antibody

20-abx101412
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

20-abx101413
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 6 (TUBB6) Antibody

20-abx101414
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

abx127119-50ul 50 ul
EUR 493.2

Tubulin Beta 1 (TUBB1) Antibody

20-abx129118
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody

20-abx123449
  • EUR 710.40
  • EUR 1028.40
  • EUR 260.40
  • EUR 493.20
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Tubulin Beta 6 (TUBB6) Antibody

abx340217-100ug 100 ug
EUR 469.2

Tubulin Beta 3 (TUBB3) Antibody

abx431689-200ul 200 ul
EUR 460.8

Tubulin Beta 1 (TUBB1) Antibody

20-abx005943
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Tubulin Beta 3 (TUBB3) Antibody

20-abx002044
  • EUR 493.20
  • EUR 710.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

Tubulin Beta 3 (TUBB3) Antibody

abx012294-100ul 100 ul
EUR 493.2

Tubulin Beta 1 (TUBB1) Antibody

abx145776-100ug 100 ug
EUR 469.2

Tubulin Beta 6 (TUBB6) Antibody

20-abx302860
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 2B (TUBB2B) Antibody

20-abx137361
  • EUR 844.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 20 ug
  • 5 ug

Tubulin Beta 2A (TUBB2A) Antibody

20-abx246726
  • EUR 627.60
  • EUR 343.20
  • 200 ul
  • 50 ul

Tubulin, Beta 2B (TUBB2B) Antibody

20-abx116350
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Tubulin Beta 2A (TUBB2A) Antibody

20-abx326997
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Tubulin Beta 2A (TUBB2A) Antibody

20-abx109386
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 2B (TUBB2B) Antibody

abx026514-400ul 400 ul
EUR 627.6

Tubulin Beta 2B (TUBB2B) Antibody

abx026514-80l 80 µl
EUR 343.2

Tubulin Beta 2A (TUBB2A) Antibody

abx027572-400ul 400 ul
EUR 627.6

Tubulin Beta 2A (TUBB2A) Antibody

abx027572-80l 80 µl
EUR 343.2

Tubulin Beta 2A (TUBB2A) Antibody

abx016016-100ug 100 ug
EUR 493.2

Tubulin Beta 2B (TUBB2B) Antibody

abx145775-100ug 100 ug
EUR 469.2

Tubulin Beta 2A (TUBB2A) Antibody

abx145790-100ug 100 ug
EUR 469.2

beta IV Tubulin antibody

22498-100ul 100ul
EUR 468

Tubulin beta 1 Antibody (TUBB1)

F51482-0.08ML 0.08 ml
EUR 140.25
Description: Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Tubulin beta-1 chain (TUBB1) is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release.

Tubulin beta 1 Antibody (TUBB1)

F40185-0.08ML 0.08 ml
EUR 140.25
Description: This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Tubulin beta-1 chain is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.

Tubulin beta 1 Antibody (TUBB1)

F40189-0.08ML 0.08 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

TUBB6 Antibody (Tubulin beta 6)

F41790-0.08ML 0.08 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

TUBB6 Antibody (Tubulin beta 6)

F41790-0.4ML 0.4 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

Tubulin beta 8 Antibody (TUBB8)

F42844-0.08ML 0.08 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

TUBB8 Antibody (Tubulin beta 8)

F42844-0.4ML 0.4 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

Tubulin beta 3 Antibody (TUBB3)

F49314-0.08ML 0.08 ml
EUR 140.25
Description: Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Tubulin beta 3 Antibody / TUBB3

R32611 100 ug
EUR 356.15
Description: Tubulin beta-3 chain is a protein that in humans is encoded by the TUBB3 gene. This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

Lenti ORF clone of Tubb4a (Myc-DDK-tagged ORF) - Rat tubulin, beta 4 (Tubb4), (10 ug)

RR203612L3 10 µg Ask for price

Beta Tubulin Antibody / TUBB

F54148-0.05ML 0.05 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Beta Tubulin Antibody / TUBB

F54148-0.2ML 0.2 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Beta Tubulin Antibody / TUBB

RQ5179 100ul
EUR 356.15
Description: The TUBB gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [RefSeq]

Beta Tubulin Antibody / TUBB

RQ6081 100 ug
EUR 356.15
Description: Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

beta 3-Tubulin (TUBB3) Antibody

20-abx134677
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

beta 3-Tubulin (TUBB3) Antibody

20-abx134682
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

beta 1-Tubulin (TUBB1) Antibody

20-abx134686
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

beta 1-Tubulin (TUBB1) Antibody

20-abx134687
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin beta 4Q Antibody (TUBB4Q)

F52213-0.08ML 0.08 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

TUBB4Q Antibody (Tubulin beta 4Q)

F52213-0.4ML 0.4 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

Tubulin beta 2A Antibody / TUBB2A

F54147-0.05ML 0.05 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Tubulin beta 2A Antibody / TUBB2A

F54147-0.2ML 0.2 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

TUBB8B Antibody (Tubulin beta 8B)

F41793-0.08ML 0.08 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

TUBB8B Antibody (Tubulin beta 8B)

F41793-0.4ML 0.4 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

TUBB2B Antibody (Tubulin beta 2B)

F41797-0.08ML 0.08 ml
EUR 140.25
Description: The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq].

TUBB2B Antibody (Tubulin beta 2B)

F41797-0.4ML 0.4 ml
EUR 322.15
Description: The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq].

Tubulin beta 2A Antibody (TUBB2A)

F43212-0.08ML 0.08 ml
EUR 140.25
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

TUBB2A Antibody (Tubulin beta 2A)

F43212-0.4ML 0.4 ml
EUR 322.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

Tubulin beta 2C Antibody (TUBB2C)

F49034-0.08ML 0.08 ml
EUR 140.25
Description: TUBB2C is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

TUBB2C Antibody (Tubulin beta 2C)

F49034-0.4ML 0.4 ml
EUR 322.15
Description: TUBB2C is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Beta III Tubulin Antibody (TUBB3)

F49314-0.4ML 0.4 ml
EUR 322.15
Description: Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Lenti ORF particles, Tubb4a (GFP-tagged) - Mouse tubulin, beta 4 (Tubb4), 200ul, >10^7 TU/mL

MR207086L4V 200 µl Ask for price

Tubulin Beta (TUBB) Antibody

abx246727-100ul 100 ul
EUR 627.6

Tubulin Beta (TUBB) Antibody

abx246728-100ul 100 ul
EUR 627.6

Tubulin Beta (TUBB) Antibody

abx246729-100ul 100 ul
EUR 627.6

Tubulin Beta (TUBB) Antibody

abx246734-400ul 400 ul
EUR 678

Tubulin Beta (TUBB) Antibody

abx246734-80l 80 µl
EUR 360

Tubulin Beta (TUBB) Antibody

20-abx249495
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Tubulin Beta (TUBB) Antibody

abx230879-100ug 100 ug
EUR 610.8

Tubulin Beta (TUBB) Antibody

abx230880-100ug 100 ug
EUR 610.8

Tubulin Beta (TUBB) Antibody

abx219172-100ug 100 ug
EUR 526.8

Tubulin Beta (TUBB) Antibody

20-abx110666
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta (TUBB) Antibody

20-abx110667
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta (TUBB) Antibody

abx139403-01mg 0.1 mg
EUR 477.6

Tubulin Beta (TUBB) Antibody

20-abx132236
  • EUR 410.40
  • EUR 1045.20
  • EUR 543.60
  • EUR 184.80
  • EUR 326.40
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta (TUBB) Antibody

20-abx132255
  • EUR 510.00
  • EUR 159.60
  • EUR 1412.40
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta (TUBB) Antibody

20-abx132364
  • EUR 393.60
  • EUR 978.00
  • EUR 510.00
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin, Beta (TUBB) Antibody

20-abx116349
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Tubulin Beta (TUBB) Antibody

20-abx118008
  • EUR 661.20
  • EUR 577.20
  • 100 ul
  • 50 ul

Tubulin Beta (TUBB) Antibody

20-abx121030
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta (TUBB) Antibody

abx025155-400ul 400 ul
EUR 627.6

Tubulin Beta (TUBB) Antibody

abx025155-80l 80 µl
EUR 343.2

Tubulin Beta (TUBB) Antibody

abx018233-100ug 100 ug
EUR 460.8

Tubulin Beta (TUBB) Antibody

abx018336-100ug 100 ug
EUR 526.8

Tubulin Beta (TUBB) Antibody

abx019025-100ug 100 ug
EUR 385.2

Tubulin Beta (TUBB) Antibody

20-abx019026
  • EUR 385.20
  • EUR 142.80
  • 100 ug
  • 10 ug

Tubulin Beta (TUBB) Antibody

abx019185-100ug 100 ug
EUR 410.4

Tubulin Beta (TUBB) Antibody

abx037893-100ug 100 ug
EUR 469.2

Tubulin Beta (TUBB) Antibody

abx037894-100ug 100 ug
EUR 469.2

Tubulin Beta (TUBB) Antibody

20-abx101054
  • EUR 493.20
  • EUR 159.60
  • EUR 1378.80
  • EUR 678.00
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta (TUBB) Antibody

20-abx124984
  • EUR 427.20
  • EUR 594.00
  • 100 ul
  • 200 ul

Tubulin Beta (TUBB) Antibody

20-abx005574
  • EUR 376.80
  • EUR 493.20
  • EUR 309.60
  • 100 ul
  • 200 ul
  • 50 ul

Tubulin Beta (TUBB) Antibody

abx012291-100ug 100 ug
EUR 526.8

Tubulin Beta (TUBB) Antibody

20-abx013240
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Tubulin Beta (TUBB) Antibody

abx145777-100ug 100 ug
EUR 469.2

Lenti ORF particles, TUBB4A (mGFP-tagged) - Human tubulin, beta 4 (TUBB4), 200ul, >10^7 TU/mL

RC203945L2V 200 µl Ask for price

Lenti ORF particles, TUBB4A (mGFP-tagged) - Human tubulin, beta 4 (TUBB4), 200ul, >10^7 TU/mL

RC203945L4V 200 µl Ask for price

Tubulin Beta 6 (TUBB6) Antibody (HRP)

20-abx311512
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody (HRP)

20-abx108851
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody (HRP)

20-abx108852
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody (HRP)

20-abx109143
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody (HRP)

20-abx310961
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 6 (TUBB6) Antibody (FITC)

20-abx311513
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBb3) Antibody (FITC)

20-abx271228
  • EUR 577.20
  • EUR 292.80
  • EUR 1696.80
  • EUR 794.40
  • EUR 427.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody (FITC)

20-abx107431
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody (FITC)

20-abx107432
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody Pair

abx117637-1pair5x96wellplates 1 pair (5x96 well plates)
EUR 1212

Tubulin Beta 1 (TUBb1) Antibody Pair

20-abx370772
  • EUR 1996.80
  • EUR 1279.20
  • 10 × 96 tests
  • 5 × 96 tests

Tubulin Beta 1 (TUBB1) Antibody (FITC)

20-abx107725
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 2A (TUBB2A) Antibody (HRP)

20-abx108850
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody (FITC)

20-abx310962
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lenti ORF particles, Tubb4a (GFP-tagged ORF) - Rat tubulin, beta 4 (Tubb4), 200ul, >10^7 TU/mL

RR203612L4V 200 µl Ask for price

Tubulin Beta 2A (TUBB2A) Antibody (FITC)

20-abx107430
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 6 (TUBB6) Antibody (Biotin)

20-abx311514
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBb3) Antibody (Biotin)

20-abx271494
  • EUR 543.60
  • EUR 292.80
  • EUR 1579.20
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody (Biotin)

20-abx106312
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody (Biotin)

20-abx106017
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 3 (TUBB3) Antibody (Biotin)

20-abx106018
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta 6 (TUBb6) Antibody (Biotin)

20-abx274250
  • EUR 560.40
  • EUR 292.80
  • EUR 1612.80
  • EUR 760.80
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Tubulin Beta 1 (TUBB1) Antibody (Biotin)

20-abx310963
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lenti ORF particles, TUBB4A (Myc-DDK tagged) - Human tubulin, beta 4 (TUBB4), 200ul, >10^7 TU/mL

RC203945L1V 200 µl Ask for price

Lenti ORF particles, TUBB4A (Myc-DDK tagged) - Human tubulin, beta 4 (TUBB4), 200ul, >10^7 TU/mL

RC203945L3V 200 µl Ask for price

Tubulin Beta 2A (TUBB2A) Antibody (Biotin)

20-abx106016
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta-8 Chain (TUBB8) Antibody

abx027305-400ul 400 ul
EUR 627.6

Tubulin Beta-8 Chain (TUBB8) Antibody

abx027305-80l 80 µl
EUR 343.2

Ocena odpowiedzi immunogennej na nowatorską szczepionkę z koniugatem enterobaktyny u kurcząt do produkcji przeciwciał żółtka jaja specyficznych dla enterobaktyny

  • Passive immunization with particular egg yolk antibodies (immunoglobulin Y, IgY) is rising as a promising various to antibiotics to manage bacterial infections. Lately, we developed a novel conjugate vaccine that might set off a robust immune response in rabbits directed towards enterobactin (Ent), a extremely conserved siderophore molecule utilized by completely different Gram-negative pathogens. Nevertheless, induction of Ent-specific antibodies seemed to be affected by the selection of animal host and vaccination routine.
  • It’s nonetheless unknown if the Ent conjugate vaccine can set off a selected immune response in layers for the aim of manufacturing of anti-Ent egg yolk IgY. On this research, three hen vaccination trials with completely different regimens have been carried out to find out circumstances for environment friendly manufacturing of anti-Ent egg yolk IgY. Purified Ent was conjugated to 3 provider proteins, keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA) and CmeC (a subunit vaccine candidate), respectively.
  • Intramuscular immunization of Barred Rock layers with KLH-Ent conjugate 4 instances induced sturdy immune response towards entire conjugate vaccine however the titer of Ent-specific IgY didn’t change in yolk with solely a four fold improve detected in serum. Within the second trial, three completely different Ent conjugate vaccines have been evaluated in Rhode Island Crimson pullets with 4 subcutaneous injections. The KLH-Ent or CmeC-Ent conjugate persistently induced excessive degree of Ent-specific IgY in each serum (as much as 2,048 fold) and yolk (as much as 1,024 fold) in every particular person hen. Nevertheless, the Ent-specific immune response was solely quickly and reasonably induced utilizing a BSA-Ent vaccination.
  • Within the third trial, ten White Leghorn layers have been subcutaneously immunized thrice with KLH-Ent, resulting in constant and robust immune response towards each entire conjugate and the Ent molecule in every hen; the imply titer of Ent-specific IgY elevated roughly 32 and 256 fold in serum and yolk, respectively. According to its potent binding to numerous Ent derivatives, the Ent-specific egg yolk IgY additionally inhibited in vitro progress of a consultant Escherichia coli pressure.
  • Collectively, this research demonstrated that the novel Ent conjugate vaccine might induce sturdy, particular, and sturdy immune response in chickens. The Ent-specific hyperimmune egg yolk IgY has potential for passive immune intervention towards Gram-negative infections.

Korelacja odpowiedzi na szczepionki

Introduction: The humoral response to vaccinations varies extensively between people. There isn’t a information obtainable on the correlation between responses to completely different vaccines. On this research, we investigated the correlation of antibody responses between routine vaccine antigens in infants.

Strategies: One and 7 months after the 6-month vaccinations and one month after the 12-month vaccinations, antibody concentrations to diphtheria, tetanus, pertussis, polio (serotypes 1-3), Haemophilus influenzae sort b (Hib), pneumococcus (13 serotypes), meningococcus C, measles, mumps and rubella have been measured utilizing fluorescent bead-based multiplex immune-assays. For the correlation of antibody responses, Spearman’s rank correlation coefficients (ρ) with 95% confidence intervals (CI) have been calculated between responses to every vaccine antigen.

Outcomes: The correlation between concentrations of antibodies to the vaccinations ending at 6 months of age was greater one month in comparison with seven months after vaccination. The strongest correlations at each time factors have been noticed between antibody responses to completely different polio serotypes, sure pneumococcal serotypes and between responses to diphtheria and pneumococcal (conjugated to a diphtheria toxoid) vaccine antigens. Correlation between responses to tetanus, Hib, pertussis, polio and different vaccine antigens have been weak. The correlation between antibody responses to the 12-month vaccine antigens was weaker than to the 6-month vaccine antigens and there was a adverse correlation between responses to measles, mumps, rubella vaccine and non-live vaccine antigens (meningococcus C, tetanus and Hib). There was solely weak correlation between antibody responses to vaccines of the identical sort (e.g. conjugated polysaccharide or toxoid vaccines).

Conclusion: Correlation between antibody responses to related antigens in the identical vaccine (equivalent to completely different serotypes of a micro organism or virus), in addition to responses to antigens conjugated to related provider proteins, are sturdy. In distinction, correlation between responses to different vaccines are weak. Measuring antibody responses to at least one or a number of vaccine antigens subsequently doesn’t supply a dependable surrogate marker of responses to unrelated vaccines.

Nowatorski automatyczny check immunologiczny dla przeciwciał NY-ESO-1 i XAGE1 w surowicy w skojarzonym przewidywaniu odpowiedzi na terapię przeciw zaprogramowanej śmierci komórkowej – 1 w niedrobnokomórkowym raku płuca

Background: Anti-programmed cell death-1 (PD-1) antibodies (Abs) are key medication in non-small-cell lung most cancers (NSCLC) remedy; nonetheless, medical advantages with anti-PD-1 monotherapy are restricted. We reported that serum Abs towards cancer-testis antigens NY-ESO-1 and XAGE1 predicted medical advantages. We aimed to develop a totally automated immunoassay system measuring NY-ESO-1/XAGE1 Abs.

Strategies: Sera from 30 NSCLC sufferers earlier than anti-PD-1 monotherapy have been reacted with recombinant NY-ESO-1 protein- or artificial XAGE1 peptide-coated magnetic beads. ALP-conjugated Ab and chemiluminescent substrate have been added and luminescence measured. These procedures have been automated utilizing excessive sensitivity chemiluminescent enzyme immunoassay (HISCLTM). NY-ESO-1/XAGE1 Ab stability was examined beneath numerous circumstances. Response prediction accuracy was evaluated utilizing space beneath receiver working curve (AUROC).

Outcomes: HISCL detected particular serum NY-ESO-1/XAGE1 Abs, which ranges in ELISA and HISCL have been extremely correlated. The Ab ranges in HISCL have been secure at 4 temperatures, 5 freeze/thaw cycles, and long-term storage; the degrees weren’t interfered by frequent blood elements. The Ab ranges in 15 NSCLC responders to anti-PD-1 monotherapy have been considerably greater than these in non-responders and wholesome donors. The AUROC was the best (0.91; 95% CI, 0.78-1.0) in combinatory prediction with NY-ESO-1/XAGE1 Abs.

Conclusion: Our immunoassay system is beneficial to foretell medical advantages with NSCLC immune-checkpoint remedy.

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