• 19 maja, 2021
bodiesrevealed

Białko mitochondrialne CMPK2 reguluje tworzenie

Białko mitochondrialne CMPK2 reguluje tworzenie komórek piankowatych wzmocnionych przez IFN alfa, potencjalnie przyczyniając się do przedwczesnej miażdżycy tętnic w SLE 

Background: Untimely atherosclerosis happens in sufferers with SLE; nonetheless, the mechanisms stay unclear. Each mitochondrial equipment and proinflammatory cytokine interferon alpha (IFN-α) probably contribute to atherogenic processes in SLE. Right here, we discover the roles of the mitochondrial protein cytidine/uridine monophosphate kinase 2 (CMPK2) in IFN-α-mediated pro-atherogenic occasions.

Strategies: Foam cell measurements have been carried out by oil purple O staining, Dil-oxLDL uptake and the BODIPY strategy. The mRNA and protein ranges have been measured by qPCR and Western blotting, respectively. Isolation of CD4+ T cells and monocytes was carried out with monoclonal antibodies conjugated with microbeads. Manipulation of protein expression was performed by both small interference RNA (siRNA) knockdown or CRISPR/Cas9 knockout. The expression of mitochondrial reactive oxygen species (mtROS) was decided by circulate cytometry and confocal microscopy.

Outcomes: IFN-α enhanced oxLDL-induced foam cell formation and Dil-oxLDL uptake by macrophages. Along with IFN-α, a number of triggers of atherosclerosis, together with thrombin and IFN-γ, can induce CMPK2 expression, which was elevated in CD4+ T cells and CD14+ monocytes remoted from SLE sufferers in comparison with these remoted from controls. The evaluation of mobile subfractions revealed that CMPK2 was current in each mitochondrial and cytosolic fractions. IFN-α-induced CMPK2 expression was inhibited by Janus kinase (JAK)half of and tyrosine kinase 2 (Tyk2) inhibitors. Each the knockdown and knockout of CMPK2 attenuated IFN-α-mediated foam cell formation, which concerned the discount of scavenger receptor class A (SR-A) expression. CMPK2 additionally regulated IFN-α-enhanced mtROS manufacturing and inflammasome activation.

Conclusions: The research means that CMPK2 performs contributing roles within the pro-atherogenic results of IFN-α.

bodiesrevealed
bodiesrevealed

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

S0006 200ul
EUR 376

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 1mg
EUR 334

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

43R-ID012AF 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 1 mg
EUR 176

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 300 Tests
EUR 306

Tubulin Beta-4A Chain (TUBB4) Antibody

abx145781-100ug 100 ug
EUR 391

Tubulin Beta-4A Chain (TUBB4) Antibody

abx145784-100ug 100 ug
EUR 391

Anti-CD40 antibody (Alexa-fluor 488)

STJ170000 100 µg
EUR 393
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-RPSA Alexa Fluor® 488

A4-829-C100 0.1 mg
EUR 310

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 448

beta IV Tubulin antibody

22498-100ul 100ul
EUR 390

Human Tubulin Beta 4(TUBb4)ELISA Kit

QY-E01098 96T
EUR 361

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 100 tests
EUR 269

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-gamma-Tubulin Alexa Fluor647

A6-465-C025 0.025 mg
EUR 217

Anti-gamma-Tubulin Alexa Fluor647

A6-465-C100 0.1 mg
EUR 394

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1233

SAM FCM (Alexa Fluor 488)

abx098904-60tests 60 tests
EUR 1358

EGFR antibody (Alexa Fluor 488)

61R-E109BAF 125 ug
EUR 706
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 0.5 ml
EUR 225

Mouse Tubulin beta- 4 chain, Tubb4 ELISA KIT

ELI-53137m 96 Tests
EUR 865

Bovine Tubulin beta- 4 chain, TUBB4 ELISA KIT

ELI-29137b 96 Tests
EUR 928

Human Tubulin beta- 4 chain, TUBB4 ELISA KIT

ELI-13731h 96 Tests
EUR 824

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 0.1 mg
EUR 269

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 100 tests
EUR 269

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Alpha Fluor™ 594 C5 Maleimide

1891 1 mg
EUR 219

Streptavidin-Alexa594 (Alexas fluor 594) conjugate

SV-A594-100 100 tests
EUR 225

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

S0013 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

S0014 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

S0017 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 200ul
EUR 304

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 10 mg
EUR 393

Anti-beta Tubulin antibody

STJ13100428 150 µl
EUR 427

Anti-Tubulin beta antibody

STJ13100435 500 µg
EUR 427

Anti-Beta-Tubulin antibody

STJ16100474 100 µg
EUR 406

Anti-Tubulin Beta antibody

STJ31562 200 µl
EUR 197
Description: Unonjugated rabbit polyclonal to tubulin beta

Anti-beta-Tubulin Purified

11-251-C025 0.025 mg
EUR 113

Anti-beta-Tubulin Purified

11-251-C100 0.1 mg
EUR 186

Anti-beta-Tubulin Purified

11-427-C025 0.025 mg
EUR 113

Anti-beta-Tubulin Purified

11-427-C100 0.1 mg
EUR 186

Anti-beta-Tubulin Purified

11-444-C025 0.025 mg
EUR 113

Anti-beta-Tubulin Purified

11-444-C100 0.1 mg
EUR 186

Anti-beta-Tubulin antibody

STJ96932 200 µl
EUR 197
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ96932 was developed from clone 1E1-E8-H4 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous beta tubulin.

Anti-beta-Tubulin antibody

STJ97016 200 µl
EUR 197
Description: Mouse monoclonal to beta-Tubulin.

Anti-beta-tubulin antibody

STJ97037 200 µl
EUR 197
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ97037 was developed from clone M7. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta tubulin.

Anti-beta-tubulin antibody

STJ97041 200 µl
EUR 197
Description: Mouse monoclonal to beta-tubulin.

Anti-beta Tubulin antibody

STJ97131 200 µl
EUR 197
Description: Rabbit polyclonal to beta Tubulin.

Anti-Tubulin beta antibody

STJ98436 100 µl
EUR 234
Description: Mouse monoclonal to Tubulin beta.

Anti-Tubulin beta antibody

STJ98566 100 µl
EUR 234
Description: Mouse monoclonal to Tubulin beta.

Anti-Tubulin beta antibody

STJ96145 200 µl
EUR 197
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ96145 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Tubulin beta protein.

Anti-beta-Tubulin antibody

STJ99132 200 µl
EUR 197
Description: Mouse monoclonal to beta-Tubulin.

Anti-beta-Tubulin antibody

STJ99133 200 µl
EUR 197
Description: Mouse monoclonal to beta-Tubulin.

anti- beta Tubulin antibody

FNab00879 100µg
EUR 548.75
Description: Antibody raised against beta Tubulin

anti- Beta Tubulin antibody

FNab00880 100µg
EUR 548.75
Description: Antibody raised against Beta Tubulin

anti- Tubulin-beta antibody

FNab09105 100µg
EUR 548.75
Description: Antibody raised against Tubulin-beta

anti- Beta Tubulin antibody

FNab09872 100µg
EUR 548.75
Description: Antibody raised against Beta Tubulin

anti-beta-Tubulin (5G3)

LF-MA20322 100 ug
EUR 334
Description: Mouse monoclonal to betA tubulin

anti-beta-tubulin (M6)

LF-MA20425 100 ug
EUR 334
Description: Mouse monoclonal to Beta tubulin

anti-beta II tubulin

LF-MA20432 100 ug
EUR 334
Description: Mouse monoclonal to beta II tubulin

anti- Beta Tubulin antibody

LSMab09872 100 ug
EUR 386

Anti-beta Tubulin antibody

PAab00879 100 ug
EUR 386

Anti-Beta Tubulin antibody

PAab00880 100 ug
EUR 386

Anti-Beta-tubulin Antibody

A1216-100
EUR 403

anti-beta II Tubulin

YF-PA23016 100 ug
EUR 403
Description: Rabbit polyclonal to beta II Tubulin

anti-beta II Tubulin

YF-PA25563 50 ul
EUR 334
Description: Mouse polyclonal to beta II Tubulin

alpha Tubulin (alpha Tubulin) Antibody (ATTO 594)

abx446844-100ug 100 ug
EUR 578

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 0.5mg
EUR 435

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 0.1 mg
EUR 269

Human Kinase Library IV

HKIN-IV 1 set
EUR 450

alpha Tubulin (alpha Tubulin) Antibody (PE/ATTO 594)

abx446854-100ug 100 ug
EUR 592

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 1 mg
EUR 176

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 1 mg
EUR 176

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 300 Tests
EUR 306

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 300 Tests
EUR 306

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Recombinant (E.Coli) Human Thyrostimulin Beta

RP-594 10 ug
EUR 286

Anti- Beta-tubulin Monoclonal Antibody

M05613-2 100ul
EUR 397
Description: Mouse Monoclonal Beta-tubulin Antibody. Validated in IF, IHC, WB and tested in Human, Monkey, Mouse, Rat.

Anti- Beta-tubulin Monoclonal Antibody

M05613-3 100ul
EUR 397
Description: Mouse Monoclonal Beta-tubulin Antibody. Validated in IHC, WB and tested in Human, Monkey, Mouse, Rat.

Anti-Tubulin, beta III antibody

STJ120262 100 µl
EUR 526

Anti-Beta III Tubulin antibody

STJ16100477 100 µg
EUR 406

Anti-Beta Tubulin/TUBB Antibody

A01857-1 100ug/vial
EUR 334

Anti-beta I tubulin antibody

STJ96939 200 µl
EUR 197
Description: beta I tubulin is a protein encoded by the TUBB1 gene which is approximately 50,3 kDa. beta I tubulin is localised to the cytoplasm. It is involved in development Slit-Robo signalling, Sertoli-Sertoli cell junction dynamics, signalling in gap junctions and chaperonin-mediated protein folding. This protein falls under the beta tubulin protein family. It is one of two core protein families that heterodimerize and assemble to form microtubules. It is exists in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. beta I tubulin is expressed in the blood and cells of the nervous system. Mutations in the TUBB1 gene may result in macrothrombocytopaenia. STJ96939 was developed from clone 3F7 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous beta I tubulin proteins.

Anti-beta I tubulin antibody

STJ97021 200 µl
EUR 197
Description: Mouse monoclonal to beta I tubulin.

Anti-beta II tubulin antibody

STJ97053 200 µl
EUR 197
Description: beta II tubulin is a protein encoded by the TUBB2A gene which is approximately 49,9 kDa. beta II tubulin is localised to the cytoplasm and cytoskeleton. It is involved in Sertoli-Sertoli cell junction dynamics, development Slit-Robo signalling and the GPCR pathway. Tubulin is the major constituent of microtubules. Microtubules are key participants in processes such as mitosis and intracellular transport and are composed of heterodimers of alpha- and beta-tubulins. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta II tubulin is expressed in the nervous system, eye, skin and lung. Mutations in the TUBB2A gene may result in lung mucoepidermoid carcinoma. STJ97053 was developed from clone Mix and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta II tubulin protein.

Anti-beta III tubulin antibody

STJ97067 200 µl
EUR 197
Description: Mouse monoclonal to beta III tubulin.

Anti-beta II Tubulin antibody

STJ97122 200 µl
EUR 197
Description: Rabbit polyclonal to beta II Tubulin.

Anti-beta III Tubulin antibody

STJ97123 200 µl
EUR 197
Description: Beta III tubulin is a protein encoded by the tubb3 gene which is approximately 50,4 kDa. Beta III tubulin is localised to the cytoplasm and cytoskeleton. It is involved in signalling within gap junctions, development slit-robo signalling, sertoli-sertoli cell junction dynamics, chaperonin-mediated protein folding and tubulin folding. This protein falls under the one of two protein families, namely beta and alpha tubulin and is one of six beta tubulin isoforms. Beta III tubulin is expressed in the neurons of the peripheral and central nervous system and plays an important role in guidance and maintenance and axons. Mutations in the tubb3 gene result in congenital fibrosis of extraocular muscles type. STJ97123 was developed from clone Mix. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta III tubulin protein.

Anti-beta-tubulin antibody (HRP)

STJ97477 200 µl
EUR 197
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ97477 was developed from clone 5G3. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta tubulin (HRP Conjugated).

anti-beta I tubulin (3F7)

LF-MA20329 100 ug
EUR 334
Description: Mouse monoclonal to beta I tubulin

Anti-beta II Tubulin (1F9)

YF-MA17277 100 ug
EUR 363
Description: Mouse monoclonal to beta II Tubulin

Anti-beta II Tubulin (1G3)

YF-MA17278 100 ug
EUR 363
Description: Mouse monoclonal to beta II Tubulin

Beta-Tubulin

MO22180 100 ug
EUR 435

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-beta Tubulin Rabbit Monoclonal Antibody

M05613 100ug/vial
EUR 397
Description: Rabbit Monoclonal beta Tubulin Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Beta Tubulin Antibody (monoclonal, 2E11)

M05613-4 100ug/vial
EUR 294

Anti-Beta Tubulin Antibody (monoclonal, 5E4)

M01857-3 100ug/vial
EUR 294

Anti- beta II tubulin Monoclonal Antibody

A06868-1 100ul
EUR 397
Description: Mouse Monoclonal Antibody for beta II tubulin Antibody (TUBB2A) detection.tested for IHC, IP, WB in Mouse, Rat.

Anti-alpha/beta-Tubulin dimer Purified

11-445-C025 0.025 mg
EUR 113

Anti-alpha/beta-Tubulin dimer Purified

11-445-C100 0.1 mg
EUR 186

Anti-alpha/beta-Tubulin dimer Purified

11-446-C025 0.025 mg
EUR 113

Anti-alpha/beta-Tubulin dimer Purified

11-446-C100 0.1 mg
EUR 186

anti-Zebrafish Specific beta-Tubulin (142)

LF-MA20407 100 ug
EUR 334
Description: Mouse monoclonal to Zebrafish Specific beta tubulin

anti-Zebrafish Specific beta I tubulin

LF-MA20412 100 ug
EUR 354
Description: Mouse monoclonal to Zebrafish Specific beta I tubulin

anti-Neuron specific beta III Tubulin

YF-PA16915 100 ug
EUR 403
Description: Rabbit polyclonal to Neuron specific beta III Tubulin

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 0.5 ml
EUR 225

Polyclonal beta Tubulin

APR15133G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human beta Tubulin. This antibody is tested and proven to work in the following applications:

Polyclonal beta Tubulin

APR15134G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human beta Tubulin. This antibody is tested and proven to work in the following applications:

beta Tubulin antibody

23176-100ul 100ul
EUR 390

beta-tubulin Antibody

abx139405-01mg 0.1 mg
EUR 398

beta-tubulin Antibody

abx139404-01mg 0.1 mg
EUR 398

β-Tubulin Antibody

39298-100ul 100ul
EUR 390

β-Tubulin Antibody

39299-100ul 100ul
EUR 390

beta Tubulin Antibody

48659-100ul 100ul
EUR 333

beta Tubulin Antibody

48659-50ul 50ul
EUR 239

Tubulin beta Antibody

AF7011 1ml
EUR 920
Description: Tubulin beta Antibody detects endogenous levels of total Tubulin beta.

beta Tubulin antibody

10R-7807 100 ug
EUR 154
Description: Mouse monoclonal beta Tubulin antibody

beta Tubulin antibody

10R-7919 100 ug
EUR 322
Description: Mouse monoclonal beta Tubulin antibody

beta Tubulin antibody

10R-1191 100 ul
EUR 316
Description: Mouse monoclonal beta Tubulin antibody

Tubulin beta Antibody

ABF7011 100 ug
EUR 438

Beta tubulin antibody

10R-10448 100 ug
EUR 435
Description: Mouse monoclonal Beta tubulin antibody

Beta tubulin antibody

10R-10449 100 ug
EUR 435
Description: Mouse monoclonal Beta tubulin antibody

Tubulin beta antibody

70R-30821 100 ug
EUR 327
Description: Rabbit polyclonal Tubulin beta antibody

Beta tubulin antibody

70R-32842 50 ug
EUR 165
Description: Rabbit polyclonal Beta tubulin antibody

beta Tubulin antibody

70R-12567 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal beta Tubulin antibody

beta Tubulin antibody

70R-13069 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal beta Tubulin antibody

Beta Tubulin antibody

70R-6025 50 ug
EUR 467
Description: Rabbit polyclonal Beta Tubulin antibody raised against the N terminal of TUBB

beta Tubulin antibody

20R-TR017 100 ug
EUR 511
Description: Rabbit polyclonal beta Tubulin antibody

Beta Tubulin antibody

PAab09872 100 ug
EUR 386

beta-Tubulin Antibody

T0023 1ml
EUR 920
Description: beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.

Tubulin beta Antibody

T0034 1ml
EUR 920
Description: Theβ-tubulin antibody can detects Zebrafish endogenousβ-tubulin protein.

TUBB4 antibody

10R-6183 100 ul
EUR 691
Description: Mouse monoclonal TUBB4 antibody

TUBB4 antibody

10R-6184 100 ul
EUR 691
Description: Mouse monoclonal TUBB4 antibody

TUBB4 antibody

10R-6185 100 ul
EUR 691
Description: Mouse monoclonal TUBB4 antibody

TUBB4 antibody

10R-6186 100 ul
EUR 691
Description: Mouse monoclonal TUBB4 antibody

TUBB4 antibody

10R-6187 100 ul
EUR 572
Description: Mouse monoclonal TUBB4 antibody

TUBB4 antibody

10R-6188 100 ul
EUR 691
Description: Mouse monoclonal TUBB4 antibody

Anti-Cytokeratins Alexa Fluor488

A4-108-C025 0.025 mg
EUR 175

Anti-Cytokeratins Alexa Fluor488

A4-108-C100 0.1 mg
EUR 310

Anti-PSMA Alexa Fluor488

A4-539-C025 0.025 mg
EUR 227

Anti-PSMA Alexa Fluor488

A4-539-C100 0.1 mg
EUR 414

Anti-FoxP3 Alexa Fluor488

A4-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor488

A4-601-C100 0.1 mg
EUR 362

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C025 0.025 mg
EUR 154

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C100 0.1 mg
EUR 269

Anti-LCK Alexa Fluor647

A6-269-C025 0.025 mg
EUR 206

Anti-LCK Alexa Fluor647

A6-269-C100 0.1 mg
EUR 373

Anti-FoxP3 Alexa Fluor647

A6-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor647

A6-601-C100 0.1 mg
EUR 362

Anti-beta I Tubulin Rabbit Monoclonal Antibody

M05397 100ug/vial
EUR 397
Description: Rabbit Monoclonal beta I Tubulin Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Canine, Cow, Human, Monkey, Mouse, Rat.

Anti-beta II Tubulin Rabbit Monoclonal Antibody

M09008 100ug/vial
EUR 397
Description: Rabbit Monoclonal beta II Tubulin Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-beta-Tubulin Antibody (monoclonal, TUB 2.1)

MA1112 100ug/vial
EUR 334

Anti-beta III Tubulin Rabbit Monoclonal Antibody

M01857 100ug/vial
EUR 397
Description: Rabbit Monoclonal beta III Tubulin Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-beta III Tubulin Rabbit Monoclonal Antibody

M01857-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal beta III Tubulin Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Recombinant Tubulin Beta (TUBb)

4-RPB870Hu01
  • EUR 512.16
  • EUR 240.00
  • EUR 1645.60
  • EUR 615.20
  • EUR 1130.40
  • EUR 406.00
  • EUR 3964.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Tubulin Beta expressed in: E.coli

Active Tubulin Beta (TUBb)

4-APB870Hu01
  • EUR 841.89
  • EUR 328.00
  • EUR 2882.08
  • EUR 1027.36
  • EUR 1954.72
  • EUR 626.00
  • EUR 7055.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Tubulin Beta expressed in: E.coli

Monoclonal beta Tubulin Antibody

APR11514G 0.1ml
EUR 484
Description: A Monoclonal antibody against Human beta Tubulin. The antibodies are raised in Mouse. This antibody is applicable in WB

Monoclonal beta Tubulin Antibody

APR11515G 0.1ml
EUR 484
Description: A Monoclonal antibody against Human beta Tubulin. The antibodies are raised in Mouse. This antibody is applicable in WB, ICC

Polyclonal Tubulin Beta Antibody

APR13883G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Tubulin Beta . This antibody is tested and proven to work in the following applications:

Tubulin beta-III Antibody

21617-100ul 100ul
EUR 252

Tubulin beta-III Antibody

21617-50ul 50ul
EUR 187

beta Tubulin 3 antibody

23104-100ul 100ul
EUR 390

Tubulin beta-8 antibody

23182-100ul 100ul
EUR 390

Tubulin, Beta (TUBB) Antibody

20-abx116349
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Tubulin Beta (TUBB) Antibody

20-abx118008
  • EUR 551.00
  • EUR 481.00
  • 100 ul
  • 50 ul

Tubulin Beta (TUBB) Antibody

20-abx121030
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Tubulin Beta (TUBB) Antibody

20-abx110666
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Tubulin Beta (TUBB) Antibody

20-abx110667
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ocena odpowiedzi immunogennej na nowatorską szczepionkę z koniugatem enterobaktyny u kurcząt do produkcji przeciwciał żółtka jaja specyficznych dla enterobaktyny

  • Passive immunization with particular egg yolk antibodies (immunoglobulin Y, IgY) is rising as a promising various to antibiotics to manage bacterial infections. Lately, we developed a novel conjugate vaccine that might set off a robust immune response in rabbits directed towards enterobactin (Ent), a extremely conserved siderophore molecule utilized by completely different Gram-negative pathogens. Nevertheless, induction of Ent-specific antibodies seemed to be affected by the selection of animal host and vaccination routine.
  • It’s nonetheless unknown if the Ent conjugate vaccine can set off a selected immune response in layers for the aim of manufacturing of anti-Ent egg yolk IgY. On this research, three hen vaccination trials with completely different regimens have been carried out to find out circumstances for environment friendly manufacturing of anti-Ent egg yolk IgY. Purified Ent was conjugated to 3 provider proteins, keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA) and CmeC (a subunit vaccine candidate), respectively.
  • Intramuscular immunization of Barred Rock layers with KLH-Ent conjugate 4 instances induced sturdy immune response towards entire conjugate vaccine however the titer of Ent-specific IgY didn’t change in yolk with solely a four fold improve detected in serum. Within the second trial, three completely different Ent conjugate vaccines have been evaluated in Rhode Island Crimson pullets with 4 subcutaneous injections. The KLH-Ent or CmeC-Ent conjugate persistently induced excessive degree of Ent-specific IgY in each serum (as much as 2,048 fold) and yolk (as much as 1,024 fold) in every particular person hen. Nevertheless, the Ent-specific immune response was solely quickly and reasonably induced utilizing a BSA-Ent vaccination.
  • Within the third trial, ten White Leghorn layers have been subcutaneously immunized thrice with KLH-Ent, resulting in constant and robust immune response towards each entire conjugate and the Ent molecule in every hen; the imply titer of Ent-specific IgY elevated roughly 32 and 256 fold in serum and yolk, respectively. According to its potent binding to numerous Ent derivatives, the Ent-specific egg yolk IgY additionally inhibited in vitro progress of a consultant Escherichia coli pressure.
  • Collectively, this research demonstrated that the novel Ent conjugate vaccine might induce sturdy, particular, and sturdy immune response in chickens. The Ent-specific hyperimmune egg yolk IgY has potential for passive immune intervention towards Gram-negative infections.

Korelacja odpowiedzi na szczepionki

Introduction: The humoral response to vaccinations varies extensively between people. There isn’t a information obtainable on the correlation between responses to completely different vaccines. On this research, we investigated the correlation of antibody responses between routine vaccine antigens in infants.

Strategies: One and 7 months after the 6-month vaccinations and one month after the 12-month vaccinations, antibody concentrations to diphtheria, tetanus, pertussis, polio (serotypes 1-3), Haemophilus influenzae sort b (Hib), pneumococcus (13 serotypes), meningococcus C, measles, mumps and rubella have been measured utilizing fluorescent bead-based multiplex immune-assays. For the correlation of antibody responses, Spearman’s rank correlation coefficients (ρ) with 95% confidence intervals (CI) have been calculated between responses to every vaccine antigen.

Outcomes: The correlation between concentrations of antibodies to the vaccinations ending at 6 months of age was greater one month in comparison with seven months after vaccination. The strongest correlations at each time factors have been noticed between antibody responses to completely different polio serotypes, sure pneumococcal serotypes and between responses to diphtheria and pneumococcal (conjugated to a diphtheria toxoid) vaccine antigens. Correlation between responses to tetanus, Hib, pertussis, polio and different vaccine antigens have been weak. The correlation between antibody responses to the 12-month vaccine antigens was weaker than to the 6-month vaccine antigens and there was a adverse correlation between responses to measles, mumps, rubella vaccine and non-live vaccine antigens (meningococcus C, tetanus and Hib). There was solely weak correlation between antibody responses to vaccines of the identical sort (e.g. conjugated polysaccharide or toxoid vaccines).

Conclusion: Correlation between antibody responses to related antigens in the identical vaccine (equivalent to completely different serotypes of a micro organism or virus), in addition to responses to antigens conjugated to related provider proteins, are sturdy. In distinction, correlation between responses to different vaccines are weak. Measuring antibody responses to at least one or a number of vaccine antigens subsequently doesn’t supply a dependable surrogate marker of responses to unrelated vaccines.

Nowatorski automatyczny check immunologiczny dla przeciwciał NY-ESO-1 i XAGE1 w surowicy w skojarzonym przewidywaniu odpowiedzi na terapię przeciw zaprogramowanej śmierci komórkowej – 1 w niedrobnokomórkowym raku płuca

Background: Anti-programmed cell death-1 (PD-1) antibodies (Abs) are key medication in non-small-cell lung most cancers (NSCLC) remedy; nonetheless, medical advantages with anti-PD-1 monotherapy are restricted. We reported that serum Abs towards cancer-testis antigens NY-ESO-1 and XAGE1 predicted medical advantages. We aimed to develop a totally automated immunoassay system measuring NY-ESO-1/XAGE1 Abs.

Strategies: Sera from 30 NSCLC sufferers earlier than anti-PD-1 monotherapy have been reacted with recombinant NY-ESO-1 protein- or artificial XAGE1 peptide-coated magnetic beads. ALP-conjugated Ab and chemiluminescent substrate have been added and luminescence measured. These procedures have been automated utilizing excessive sensitivity chemiluminescent enzyme immunoassay (HISCLTM). NY-ESO-1/XAGE1 Ab stability was examined beneath numerous circumstances. Response prediction accuracy was evaluated utilizing space beneath receiver working curve (AUROC).

Outcomes: HISCL detected particular serum NY-ESO-1/XAGE1 Abs, which ranges in ELISA and HISCL have been extremely correlated. The Ab ranges in HISCL have been secure at 4 temperatures, 5 freeze/thaw cycles, and long-term storage; the degrees weren’t interfered by frequent blood elements. The Ab ranges in 15 NSCLC responders to anti-PD-1 monotherapy have been considerably greater than these in non-responders and wholesome donors. The AUROC was the best (0.91; 95% CI, 0.78-1.0) in combinatory prediction with NY-ESO-1/XAGE1 Abs.

Conclusion: Our immunoassay system is beneficial to foretell medical advantages with NSCLC immune-checkpoint remedy.

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