Human alpha 1 Antichymotrypsin ELISA Kit
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13 ng/ml
- Range: 31.25 ng/ml – 2000 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay Type: Sandwich (quantitative)
- Reacts with: Human
Product description:
The Human Alpha 1 Antitrypsin (SERPINA1) ELISA Kit (ab189579) is a 90 minute single wash sandwich ELISA designed for the quantitative measurement of Alpha 1 antitrypsin protein (SERPINA1) in cit plasma, liver plasma, serum and edta plasma. . It uses our patented SimpleStep ELISA® technology. Quantify human alpha 1 antitrypsin (SERPINA1) with a sensitivity of 13 ng / ml.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This sandwich ELISA approach allows for the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the imaging section for more details. Our SimpleStep ELISA® technology offers several benefits:
– Single wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity and reproducibility of superior antibodies
– Fully validated in biological samples
– 96-well plate that can be broken into 12 x 8-well strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available for use as an alternative to the 96-well microplate provided with the SimpleStep ELISA® kits.
TEST SPECIFICITY:
This kit recognizes native and recombinant human alpha 1 antitrypsin protein in serum, plasma and cell culture supernatant, cell extract samples and tissues only.
Normal human urine and saliva samples were negative for this kit.
CROSS REACTIVITY:
Human alpha 1 anti-chymotrypsin protein and human PAI protein were prepared at 2000 ng/ml and 1000 ng/ml respectively and tested for cross-reactivity. No cross-reactivity was observed.
INTERFERENCE
Human alpha 1 anti-chymotrypsin protein and human PAI protein were prepared at 2000 ng/ml and 1000 ng/ml respectively and analyzed and analyzed for interference. No interference was observed.
REACTIVITY OF THE SPECIES:
This kit recognizes human alpha 1 antitrypsin protein.
The reactivity of other species was determined by measuring serum samples of alpha 1 antitrypsin (1: 10,000) from various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum. tested at the same dilution.
A reactivity <3% was determined for the following species: mouse, rat, hamster, guinea pig, rabbit, dog, goat, pig and cow.
CALIBRATION:
This immunoassay is calibrated against a highly purified human alpha 1 antitrypsin. NIBSC / WHO purified human alpha 1 antitrypsin preparation 05/162 was tested in this kit.
The dose-response curve for unclassified alpha 1 antitrypsin standard parallels the SimpleStep standard curve. To convert the sample values obtained with the SimpleStep Human alpha 1 Antitrypsin Kit to approximate NIBSC 05/162 units, use the following equation.
Notes:
Alpha 1 antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, its primary target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in insulin-induced NO synthesis and clotting. Alpha 1 antitrypsin deficiency causes emphysema and can also lead to liver disease.
