• April 20, 2021

Human alpha 1 Antichymotrypsin ELISA Kit

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 13 ng/ml
  • Range: 31.25 ng/ml – 2000 ng/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay Type: Sandwich (quantitative)
  • Reacts with: Human

Product description:

The Human Alpha 1 Antitrypsin (SERPINA1) ELISA Kit (ab189579) is a 90 minute single wash sandwich ELISA designed for the quantitative measurement of Alpha 1 antitrypsin protein (SERPINA1) in cit plasma, liver plasma, serum and edta plasma. . It uses our patented SimpleStep ELISA® technology. Quantify human alpha 1 antitrypsin (SERPINA1) with a sensitivity of 13 ng / ml.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This sandwich ELISA approach allows for the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the imaging section for more details. Our SimpleStep ELISA® technology offers several benefits:

– Single wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity and reproducibility of superior antibodies
– Fully validated in biological samples
– 96-well plate that can be broken into 12 x 8-well strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available for use as an alternative to the 96-well microplate provided with the SimpleStep ELISA® kits.

TEST SPECIFICITY:

This kit recognizes native and recombinant human alpha 1 antitrypsin protein in serum, plasma and cell culture supernatant, cell extract samples and tissues only.

Normal human urine and saliva samples were negative for this kit.

CROSS REACTIVITY:

Human alpha 1 anti-chymotrypsin protein and human PAI protein were prepared at 2000 ng/ml and 1000 ng/ml respectively and tested for cross-reactivity. No cross-reactivity was observed.

INTERFERENCE

Human alpha 1 anti-chymotrypsin protein and human PAI protein were prepared at 2000 ng/ml and 1000 ng/ml respectively and analyzed and analyzed for interference. No interference was observed.

REACTIVITY OF THE SPECIES:

This kit recognizes human alpha 1 antitrypsin protein.

The reactivity of other species was determined by measuring serum samples of alpha 1 antitrypsin (1: 10,000) from various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum. tested at the same dilution.
A reactivity <3% was determined for the following species: mouse, rat, hamster, guinea pig, rabbit, dog, goat, pig and cow.

CALIBRATION:

This immunoassay is calibrated against a highly purified human alpha 1 antitrypsin. NIBSC / WHO purified human alpha 1 antitrypsin preparation 05/162 was tested in this kit.

The dose-response curve for unclassified alpha 1 antitrypsin standard parallels the SimpleStep standard curve. To convert the sample values ​​obtained with the SimpleStep Human alpha 1 Antitrypsin Kit to approximate NIBSC 05/162 units, use the following equation.

Notes:

Alpha 1 antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, its primary target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in insulin-induced NO synthesis and clotting. Alpha 1 antitrypsin deficiency causes emphysema and can also lead to liver disease.

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