QAYEE-BIO For life science
Key Features and Details
- Sensitivity: 10 pg / ml
- Range: 78 pg / ml – 5000 pg / ml
- Sample type: cell culture supernatant, cell lysate, EDTA plasma, liver plasma, serum
- Detection method: colorimetric
- Test type: Sandwich (quantitative)
- Reacts with: Human
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) Kit (ab213839) is designed for the quantitative measurement of human TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard enzyme-linked immunosorbent sandwich assay technology. A mouse monoclonal antibody specific for BAFF-R has been precoated in 96-well plates. Standards (expression system for standard: NSO; immunogen sequence: S7-A71) and test samples are added to the wells, a goat biotinylated polyclonal detection antibody specific for BAFF-R is subsequently added, and then wash with PBS or TBS buffer.
The avidin-biotin-peroxidase complex is added and unbound conjugates are washed with PBS or TBS buffer. The HRP TMB substrate is used to visualize the enzymatic reaction of HRP. TMB is catalyzed by HRP to produce a blue product that changes to yellow after adding an acid stop solution. The density of yellow is proportional to the amount of human BAFF-R sample captured on the plate.
BAFF-R (also known as member 13C of the tumour necrosis factor receptor or B cell-activating factor receptor superfamily) is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31.
B cell-activating factor (BAFF) improves B cell survival in vitro and is a regulator of the peripheral B cell population. The protein encoded by this gene is a BAFF receptor and is a type III transmembrane protein that contains a single phenylalanine-rich extracellular domain. This receptor is believed to be the major receptor required for the BAFF-mediated survival of mature B cells.